Journal: STAR Protocols

Article Title: Protocol for generating human craniofacial cartilage organoids from stem-cell-derived neural crest cells

doi: 10.1016/j.xpro.2024.103532

Figure Lengend Snippet:

Article Snippet: Use EasySep Release Human PSC-derived Neural Crest Cell Positive Selection Kit, Stem cell Technologies, Cat# 100–047 to sort the cells based on cell surface CD271 (NGFR) marker using magnetic beads as described in the following attached kit manufacturer’s protocol: https://cdn.stemcell.com/media/files/pis/10000007951-PIS_00.pdf . j.

Techniques: Recombinant, Knock-Out, Membrane, Selection

Journal: Modern Technologies in Medicine

Article Title: Cytokine Profile of CCR6 + T-Helpers Isolated from the Blood of Patients with Peptic Ulcer Associated with Helicobacter pylori Infection

doi: 10.17691/stm2020.12.3.04

Figure Lengend Snippet: (a) PBMCs were cultured without activation or activated with PMA and ionomycin for 4 or 22 h. T-helpers were gated as CD4 + lymphocytes. From composition of this subpopulation, CD45RO + T-helpers were selected in gate P3 and CCR6 + T-helpers were selected in gate P4. The numbers of gated cells are shown as percentages of all T-helpers. The expression of IFN-g and IL-17A in CD45RO + T-helper cells is shown in the right columns of dotplots. The proportion of IFN-g + IL-17А – cells (%) is indicated in the Q1 quadrants, that of IFN-g + IL-17A + — in Q2, and that of IFN-g – IL-17A + — in Q4; (b) expression of IFN-g and IL-17A upon 4-hour activation of PBMCs and subsequent gating of T-helper cells of different phenotypes (phenotypes indicated above the graphs)

Article Snippet: Then, 9 μl of the EasySep Human CCR6 + Positive Selection Cocktail II reagent (STEMCELL Technologies) was added to link the CCR6 to the bead surface.

Techniques: Cell Culture, Activation Assay, Expressing

Journal: Modern Technologies in Medicine

Article Title: Cytokine Profile of CCR6 + T-Helpers Isolated from the Blood of Patients with Peptic Ulcer Associated with Helicobacter pylori Infection

doi: 10.17691/stm2020.12.3.04

Figure Lengend Snippet: (a) phenotypes of cells obtained at the stages of purification; phenotype of the final purification product — mature CCR6 + T-helpers — is framed; (b) staining control of PBMCs and final product of purification (in the frame). The cells are sequentially gated: lymphocytes — in gate R1, then CD4 + lymphocytes — in gate R2, then CD4 + CD45RO + CCR6 + lymphocytes — in gate R3. The quantity of gated cells is indicated as percent of the total number of lymphocytes

Article Snippet: Then, 9 μl of the EasySep Human CCR6 + Positive Selection Cocktail II reagent (STEMCELL Technologies) was added to link the CCR6 to the bead surface.

Techniques: Purification, Staining, Control

Journal: Modern Technologies in Medicine

Article Title: Cytokine Profile of CCR6 + T-Helpers Isolated from the Blood of Patients with Peptic Ulcer Associated with Helicobacter pylori Infection

doi: 10.17691/stm2020.12.3.04

Figure Lengend Snippet: (a) from left to right: staining control; expression of IFN-γ and IL-17A in cells cultured for 4 h without activation; activation for 4 h; activation for 22 h; (b) proportion of cells producing IFN-γ, IL-17A, and both cytokines simultaneously, in cultures of mature CCR6 + T-helpers (CCR6 + Th) and unseparated T-helpers (all Th) activated within 4 h after 18 or 114 h of pre-incubation; (c) expression of IFN-γ and IL-17A in mature CCR6 + T-helpers ( left pair of graphs ) and unseparated T-helpers ( right pair of graphs ) after 114 h-pre-incubation and 4 h-activation. Top panel s : staining control

Article Snippet: Then, 9 μl of the EasySep Human CCR6 + Positive Selection Cocktail II reagent (STEMCELL Technologies) was added to link the CCR6 to the bead surface.

Techniques: Staining, Control, Expressing, Cell Culture, Activation Assay, Incubation

Journal: Modern Technologies in Medicine

Article Title: Cytokine Profile of CCR6 + T-Helpers Isolated from the Blood of Patients with Peptic Ulcer Associated with Helicobacter pylori Infection

doi: 10.17691/stm2020.12.3.04

Figure Lengend Snippet: (a) yield of mature CCR6 + T-helpers from 1 ml of blood; (b) proportion of cytokine producers in cultures of mature CCR6 + T-helper; (c) yield of cytokine-producing mature CCR6 + T-helpers from 1 ml of blood. The combination of produced cytokines is shown at the bottom; * significantly different from healthy donors (p<0.05)

Article Snippet: Then, 9 μl of the EasySep Human CCR6 + Positive Selection Cocktail II reagent (STEMCELL Technologies) was added to link the CCR6 to the bead surface.

Techniques: Produced